Namaste, iam Diane Beckman, G’day, mate.

How Much Internal Standard Should I Add? [Solved]

A good rule of thumb is to target the internal standard to the lower 1/3 of the working standard curve. This is a range that will give a comfortable response without interfering with the ionization of the analyte, see Figure 1.19 Jan 2016

Internal Standards

The method of

Internal standards

Corrections: At 2 minutes 35 seconds, I accidentally moved the wrong math over. It

Standard addition & Internal standard || External & Internal standard

Don’t miss this lecture about how to quantify concentration of unknown target analytes in samples by having